Journal: Neurochemical Research

Article Title: VCAM-1 + Mesenchymal Stem/Stromal Cells Reveal Preferable Efficacy Upon an Experimental Autoimmune Encephalomyelitis Mouse Model of Multiple Sclerosis Over the VCAM-1 − Counterpart

doi: 10.1007/s11064-024-04267-w

Figure Lengend Snippet: Glial cells in EAE mice were consistently suppressed by VCAM-1− and VCAM-1 + hUC-MSCs a – b Representative IHC staining ( a ) and statistical analysis ( b ) showed the distribution of Gfap + astrocytes and Iba + microglia in spinal cord of mice in the indicated groups (C, E, U, V). Scale bar = 20 μm. c – d Representative IF staining ( c ) and statistical analysis ( d ) showed the distribution of Gfap + astrocytes and Iba + microglia in brain of mice in the indicated groups (C, E, U, V). Scale bar = 20 μm. Data were shown as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001; ns, not significant

Article Snippet: The expression of neural and inflammatory biomarkers was detected by immunohistochemical (IHC) staining (Zsbio, Beijing, China) with the indicated antibodies (Iba, Gfap, CD45, CD68, Olig2).

Techniques: Immunohistochemistry, Staining

Journal: Neurochemical Research

Article Title: VCAM-1 + Mesenchymal Stem/Stromal Cells Reveal Preferable Efficacy Upon an Experimental Autoimmune Encephalomyelitis Mouse Model of Multiple Sclerosis Over the VCAM-1 − Counterpart

doi: 10.1007/s11064-024-04267-w

Figure Lengend Snippet: Inflammatory infiltrates in CNS of EAE mice was effectively alleviated by VCAM-1 − and VCAM-1 + hUC-MSCs infusion a Representative H&E staining showed the inflammatory infiltrates in the spinal cord of mice in the indicated groups (C, E, U, V). Scale bar = 20 μm. b Immunohistochemical (IHC) staining showed the content of CD45 + leukocytes and CD68 + macrophages in the spinal cord of mice in the aforementioned groups. Scale bar = 20 μm. c IHC staining showed the content of CD45 + leukocytes and CD68 + macrophages in the brain of mice in the aforementioned groups. Scale bar = 20 μm. d – e Statistical analysis of CD45 + area and CD68 + area in the spinal cord (d) and brain ( e ) of mice in the aforementioned groups. f – g qRT-PCR analysis showed the mRNA expression of proinflammatory factors (Ifn-γ, Tnf-α, Il-6, Il-1β) in spinal cord ( f ) and brain ( g ) of mice in the aforementioned groups. Data were shown as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; ns, not significant

Article Snippet: The expression of neural and inflammatory biomarkers was detected by immunohistochemical (IHC) staining (Zsbio, Beijing, China) with the indicated antibodies (Iba, Gfap, CD45, CD68, Olig2).

Techniques: Staining, Immunohistochemical staining, Immunohistochemistry, Quantitative RT-PCR, Expressing

Journal: Heliyon

Article Title: Lutein improves remyelination by reducing of neuroinflammation in C57BL/6 mouse models of multiple sclerosis

doi: 10.1016/j.heliyon.2024.e39253

Figure Lengend Snippet: A) Olig2 marker immunohistochemistry staining. B) Frequency of oligodendrocytes (Olig2 + population) in the corpus callosum region in different groups. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001significant vs. the cuprizone group; #P < 0.05, ##P < 0.01, ###P < 0.001, and ####P < 0.0001 significant vs. the DMF group (n = 6).

Article Snippet: Rabbit polyclonal anti-olig2 antibodies (NBP1-28667, dilution = 1:800) were used as primary antibodies, and staining procedures were carried out following the supplier's instructions (Novus Biologicals, USA).

Techniques: Marker, Immunohistochemistry, Staining